Having successfully amplified the NRAS gene from wild-type and cancerous tissue through PCR,
you now sent your PCR product for sequencing. However, the only available technology to you
is classical Sanger sequencing or the dideoxy chain termination method, explained in Figure 2.
Shown below are the sequencing gels obtained for the sections of the wild-type and mutant NRAS
genes where the mutation can be found.