The sites at which each of the 3 different enzymes will cut the same strand of lambda DNA are shown in the maps (see figure 1 B-D), each vertical line on the map is where the respective enzymes will cut.
1.1 Calculate the size of the resulting fragments at B C and D after digestion and write the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see above Figure)

1.2 How many fragments would you expect to see for each of the maps indicated in question 1 above?

1.3 Use the sequence provided and make use of figure 2 to determine what restriction enzyme uses the specific recognition site and figure 3 and 1 to determine how many times does the sequence occur in the λ DNA sequence?